Biochip Langdao (Tianjin)Medical Technology Limited

1.Product Overview

[product name] : Serum amyloid A (SAA)

[Package Specification] : 30 tests/kit;60 tests/kit

[Applicable Device] : CLite-160i

[Specimen Type] : serum

[Storage] : 2-8℃

[Product validity] : 12 months

[Detection method] : magnetic particle chemiluminescence method

2.technique principle

Double antibody sandwich method was used in the kit. The detection principle is: the sample to be tested, R1 reagent, R2 reagent and M reagent are mixed for incubation, and the fluorescein (FITC) labeled SAA antibody 1 in R1 reagent and the alkaline phosphatase (ALP) labeled SAA antibody 2 in R2 reagent are combined with the SAA antigen molecules in the sample to form an immune complex. The immune complex is further captured by anti-FITC antibodies coupled to the surface of the magnetic particle and adsorbed to the surface of the magnetic particle. After washing, the unbound material was removed and the luminescent substrate was added, ALP catalyzed the luminescence of the substrate, and the relative luminescence intensity (RLU) was determined

3.The clinical significance

1. Quickly diagnose bacterial and viral infections.

2. It can provide better differentiation. The half-life of SAA is extremely short, only about 50 minutes, which is conducive to early diagnosis and prognosis observation.

3. It is widely used in auxiliary diagnosis of infectious diseases, risk prediction of coronary heart disease, dynamic monitoring of curative effect and prognosis of tumor patients, monitoring of transplant rejection, and monitoring of improvement of rheumatoid arthritis.

4. Transplantation rejection, SAA detection is a very sensitive indicator.

5. Diagnose secondary amyloidosis.

6. Especially in the early diagnosis of children's infectious diseases and neonatal septicemia, it has greater significance than doing alone in the early identification of bacterial and viral infections in infants' infectious diseases.

7. The increase in concentrations of SAA and hs CRP is positively correlated with the potential risk of future cardiovascular events in healthy populations, and both indicators have a greater significance in predicting the risk of cardiovascular events.

Combination of SAA and CRP: In the early stage of infection, with weak inflammatory stimulation, SAA is more sensitive than CRP. SAA can increase within 5-6 hours of viral infection, and is used to distinguish between bacterial and viral infections. CRP is an obvious indicator of local bacterial susceptibility, and the combination of SAA and CRP detection can better reflect complementary advantages. It has important clinical value for the preliminary diagnosis of the cause of fever and the diagnosis of the cause of infection in patients.

4.Product features and indicators

High sensitivity: the detection limit is not higher than3mg/L;

Wide linear range: samples in the range of 3.0~500.0 (mg/L) do not need to be diluted;

Good correlation: c correlation R > 0.95 with SIEMENS reagent;

Good stability: the calibration cycle can be up to 14 days, and the reagent can be stored at 4℃ for 1 year;

Fast results: only 16-18 minutes to get results.