Biochip Langdao (Tianjin)Medical Technology Limited

1.Product Overview

[Product Name] : Heart-type fatty acid Binding Protein (H-FABP)

[Package Specification] : 30 tests/kit;60 tests/kit

[Applicable Device] : CLite-160i

[Specimen Type] : plasma

[Storage] : 2-8℃

[Product validity] : 12 months

[Detection method] : magnetic particle chemiluminescence method

2.technique principle

Double antibody sandwich method was used in the kit. The detection principle is: the sample to be tested, R1 reagent, R2 reagent and M reagent are mixed for incubation, and the fluorescein (FITC) labeled FABP antibody 1 in R1 reagent and the alkaline phosphatase (ALP) labeled FABP antibody 2 in R2 reagent are combined with the FABP antigen molecules in the sample to form an immune complex. The immune complex is further captured by anti-FITC antibodies coupled to the surface of the magnetic particle and adsorbed to the surface of the magnetic particle. After washing, the unbound material was removed and the luminescent substrate was added, ALP catalyzed the luminescence of the substrate, and the relative luminescence intensity (RLU) was determined

3.Clinical significance

Heart-type fatty acid binding protein can detect early myocardial injury caused by acute coronary syndrome, and can also detect myocardial injury in patients with heart failure, viral myocarditis, trauma, and sleep apnea syndrome. It has a good value in judging the prognosis of patients with acute pulmonary embolism, heart failure, chronic thromboembolic pulmonary hypertension, and acute cerebral infarction.

4.Product features and indicators

High sensitivity: the detection limit is not higher than 2.5ng/mL;

Wide linear range: [2.5,160] (ng/mL) within the scope of sample without dilution;

Good correlation: correlation with contrast reagent R > 0.95;

Good stability: the calibration cycle can be up to 14 days, the reagent can be stored at 4℃ for 1 year;

Fast results: only 16-18 minutes to get results.